Process the FID using command “ xfb”, and phase the spectrum when necessary.Start the experiment using command " zg".Check and adjust receiver gain using the command " rga" before starting the experiment, and the rg value should be set reservedly.Check/adjust NS, DS, D1, mixing time if required Set gradient files and percentages defined in the pulse program For a 13C and 15N double labeled sample, ZGOPTNS should be set to "-DLABEL_CN" for decoupling purpose.Note that this command may not work for customized pulse programs if the pulse definitions differ from the Bruker default. This command also can be applied to other nuclei as well, such as "getprosol 13C A B". " getprosol 1H A B" command, in which A is the calibrated 90° proton pulse length, and B is the related power level, will set all pulse parameters for you based on the values provided in the command. Set power level and pulse length parameters including high power pulses, decouple pulses, shape pulses, and et al.Type " eda", to open pulse program parameter window.Define spectrum resolution by setting parameters TD F1 and TD F2.Wrong setting may result in fold-back peaks. Define/check the spectrum dimension and window position by setting parameters SW, O1P, O2P.Type " ased" to open the acquisition parameter window.If there is no exisiting experiment or data set available, and topspin has only the pulse program, then you will have to set parameters manually.For instance, " rpar HSQCETFPF3GP" will create a new 1H-15N HSQC experiment. Or, use " rpar" command to read from an existing data set.Use command " edc" to generate a new experiment set from the currently displayed experiment.Details of operation are posted next to it. This computer also has its own IP address to facilitate data transfer to another computer. Data can be transfered from Spectrometer computers to this computer for further processing and viewing in TopSpin. ICONNMR, multiple sample and multiple experiment set-upĪ Data Processing computer is available in the lab.Triple Resonance Experiments for Protein Structure Determination.Saturation Transfer Difference Experiment.CARA (Computer Aided Resonance Assignment): Project Setup and Spectrum viewing, Sequential Assignment using CARA.2D NMR Experiments: SOFAST-HMQC, HSQC, HMBC, 2D TOCSY, 2D NOESY.Additional training sessions are available upon request.NMR operation differences on CP800 comparing to 600MHz spectrometers at the facility.File structure and permission differences in Linux system.Experiment set up (2 hours, mandatory to most of new users ):.New user must demonstrate proficiency in practical NMR operation, or attend the NMR training provided by the facility.New users are strongly recommended to attend NMR courses (CHEM471, BCHM669) provided by the Department of Chemistry and Biochemistry.complete the new account info form to open an NMR account.To become a NMR user of the facility, he/she needs to